Do you have cruise control and if so, does it work? It's just that it also uses the speed signal output from the ABS ECU.
If its of any help, the wire in question is red/blue that also runs to the following places:
Xenon headlight control unit
Satnav connector socket (passenger footwell behind kick panel)
If you don't have cruise then you could access the signal on the cruise connector under the bonnet. Also use the red/blue wire, it provides a switched on/off 12v signal proportional to vehicle speed @ 1004 pulses per mile. Setting a meter on 12v DC between the red/blue and ground will see the signal switch on/off/on/off as you drive along, drive faster and the rate increases,
My point here is that if you can tap into the signal somewhere and you do get the switching on/off signal then the ECU is outputting, if not then not - if you can see my logic. You could also do a continuity test, - if you get continuity from the cruise connector back to the red/blue wire on the ECU then the signal isn't leaving the ECU, if you don't get the continuity then you may have a damaged wire somewhere.
Continuity testing is ok as long as you restrict it to JUST the red/blue wire. My money is on the ECU.
HTH ........ Rob